Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Maturation Dependent Vulnerability of Oligodendrocytes to Oxidative Stress Induced Death Caused by Glutathione Depletion Journal of Neuroscience What is glutathione and why must we protect patient access to it? NSAIDs Depletes Glutathione Essential Formulas Glutathione Understanding the Powerful Master Antioxidant Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification