Glutathione Depletion in Mitochondrial Diseases Glutathione Reporter Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. I'm Dr Ashley Mayer . I'm a practicing integrative physician, and also a mama to two great kiddos of my own! If you caught my post on Ibuprofen a few days ago, Glutathione: The Master Antioxidant and its Importance Pyroglutamic Acidemia: An Underrecognized and Underdiagnosed Cause of High Anion Gap Metabolic Acidosis A Case Report and Review of Literature Cureus Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram