Sex differences in glutathione metabolism and acetaminophen toxicity Acetaminophen metabolism Acetaminophen's Role in Autism and ADHD: A Mitochondrial Perspective How do glutathione and Nacetylcysteine (NAC) compare regarding indications, dosing, effectiveness, and safety for acetaminophen toxicity, mucolytic therapy, and general antioxidant supplementation? Metabolism of acetaminophen (paracetamol) in plantstwo independent pathways result in the formation of a glutathione and a glucose conjugate Environmental Science and Pollution Research Springer Nature Link Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.