Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Tylenol's Impact on Glutathione and Liver Damage Glutathione Depletion in Mitochondrial Diseases Glutathione Reporter Glutathione, Alcohol and drugs Glutathione, Tylenol, and Vaccines: What Parents Need to Know Lotus Homeopathy, Inc.