Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Circulating acetaminophen metabolites are toxicokinetic biomarkers of acute liver injury Vliegenthart 2017 Clinical Pharmacology & Therapeutics Wiley Online Library Data summary: IL 4 regulates glutathione levels in the liver and lung Download Scientific Diagram The multiple mechanisms and modes of cell death after acetaminophen overdose Nini Munoz , PhD. Since the administration claimed Tylenol as a cause of autism, we've seen a number of alternative and 'integrative' practitioners talking Instagram The mechanism of action of N acetylcysteine (NAC): The emerging role of H2S and sulfane sulfur species ScienceDirect