Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram Glutathione: A powerful anti oxidant The New School of Nutritional Medicine Liver specific Bcl3 Knockout Alleviates Acetaminophen induced Liver Injury by Activating Nrf2 Pathway in Male Mice Cellular and Molecular Gastroenterology and Hepatology Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity