Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Better alternatives for pain relief than Tylenol NSAIDs Turnpaugh Health & Wellness TYLENOL [Acetaminophen] metabolism A lot of GLUTATHIONE is needed to deal with the Oxidative Stress generated by the CYP 450 metabolism of Tylenol. When people go to the ER for Tylenol Overdose Taking Tylenol? The Glutathione Connection Dr. Green Mom View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism