Acetaminophen metabolism Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Can I give Tylenol (acetaminophen) to a patient with elevated aspartate aminotransferase (AST) alanine aminotransferase (ALT) levels? Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Tylenol lowers glutathione, which is why it can cause liver failure. Low glutathione can cause many serious health problems that can vary from person to person. Finding out if you have genes that can Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health