TYLENOL Proactive Support* Muscle & Joint + Stress Glutathione, Tylenol & Your Health: What You Should Know Elderly mice with history of acetaminophen intoxication display worsened cognitive impairment and persistent elevation of astrocyte and microglia burden Scientific Reports Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Tylenol's Impact on Glutathione and Liver Damage Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.