Glutathione, Alcohol and drugs Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Mini Review Acetaminophen Toxicity and 5 Oxoproline (Pyroglutamic Acid): A Tale of Two Cycles, One an ATP Depleting Futile Cycle Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification Acetaminophen Overdose Reveals PAR4 as a Low Expressing but Potent Receptor on the Hepatic Endothelium in Mice Arteriosclerosis, Thrombosis, and Vascular Biology Acetaminophen at low doses depletes airway glutathione and alters respiratory reflex responses Smith 2013 The FASEB Journal Wiley Online Library