GSH reduces NAPQI, the metabolite responsible for hepatotoxicity in acetaminophen toxicity. Toxic doses increase oxidation into NAPQI, overwhelming glutathione. How does ethanol interfere with acetaminophen metabolism? Image: https: t.co 6EY9dIwLXY Acetaminophen or Tylenol Toxicity Lect 19 Pharmacology Acetaminophen metabolism What To Avoid When Taking Glutathione Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. NSAIDs & Acetaminophen: ditki medical and biological sciences